Synthesis and characterization of water-soluble C₆₀–peptide conjugates

• Yue Ma,
• Lorenzo Persi and
• Yoko Yamakoshi

Beilstein J. Org. Chem. 2024, 20, 777–786, doi:10.3762/bjoc.20.71

• aggregations in aqueous solution, as observed by dynamic light scattering (DLS), cryoTEM, and concentration-dependent surface tension measurements [40]. Despite the small size (≈10 nm in hydrodynamic diameter), the aggregation of these fullerene moieties was not ideal for biological applications as

Study on the interactions between melamine-cored Schiff bases with cucurbit[n]urils of different sizes and its application in detecting silver ions

• Jun-Xian Gou,
• Yang Luo,
• Xi-Nan Yang,
• Wei Zhang,
• Ji-Hong Lu,
• Zhu Tao and
• Xin Xiao

Beilstein J. Org. Chem. 2021, 17, 2950–2958, doi:10.3762/bjoc.17.204

• the reason why the proton signals and absorbance of TBT in the 1H NMR and UV–vis spectra greatly declines. Then SEM and dynamic light scattering (DLS) are used for in-depth research of Q[8]-TBT complex. As shown in Figure 4b, compared with TBT (5.35 nm), the particle size of Q[8]-TBT is greatly

Constrained thermoresponsive polymers – new insights into fundamentals and applications

• Patricia Flemming,
• Alexander S. Münch,
• Andreas Fery and
• Petra Uhlmann

Beilstein J. Org. Chem. 2021, 17, 2123–2163, doi:10.3762/bjoc.17.138

• various methods, such as turbidimetry [83], 1H NMR spectrosocopy [84], and dynamic light scattering [85][86]. In this context, the cloud-point is the phase transition temperature at a certain polymer concentration, which can be located at any point on the binodal curve, and therefore, the polymer

Enhanced target cell specificity and uptake of lipid nanoparticles using RNA aptamers and peptides

• Roslyn M. Ray,
• Anders Højgaard Hansen,
• Maria Taskova,
• Bernhard Jandl,
• Jonas Hansen,
• Citra Soemardy,
• Kevin V. Morris and
• Kira Astakhova

Beilstein J. Org. Chem. 2021, 17, 891–907, doi:10.3762/bjoc.17.75

• simultaneously assemble the LNPs (the formulation list is provided in Table 1). At this stage, the LNPs were examined by dynamic light scattering (DLS, Table 2). While noncomplexed (empty) LNPs had an average size of 62.4 nm and a zeta potential (ZP) of −2.9 mV, LNPs mixed with GP160:A-1 and CCR5:G-3 displayed

Multiswitchable photoacid–hydroxyflavylium–polyelectrolyte nano-assemblies

• Alexander Zika and
• Franziska Gröhn

Beilstein J. Org. Chem. 2021, 17, 166–185, doi:10.3762/bjoc.17.17

• concentration of the polyelectrolyte and the photoacid were varied according to the anticipated concentration and loading ratios. For UV light irradiation, a UV lamp UVLM-28 EL from analytikjena with 8 W was used. Dynamic light scattering was carried out at an ALV 5000 correlator with 320 channels (ALV GmbH

• nano-assemblies for cycle I (a) and cycle II (b). Dynamic light scattering: Electric field autocorrelation function g1(τ) and distribution of relaxation times A(τ) at a scattering angle of θ = 90° for cycle I (a) and cycle II (b,c). Cycle II is separated for better distinguishability: b) the states A

Naphthalonitriles featuring efficient emission in solution and in the solid state

• Sidharth Thulaseedharan Nair Sailaja,
• Iván Maisuls,
• Jutta Kösters,
• Alexander Hepp,
• Andreas Faust,
• Jens Voskuhl and
• Cristian A. Strassert

Beilstein J. Org. Chem. 2020, 16, 2960–2970, doi:10.3762/bjoc.16.246

• environment responsiveness is based on having variable substituents at the para-position of the two phenyl moieties. The excited state properties were fully investigated in tetrahydrofuran (THF) solutions and in THF/H2O mixtures. The size of the aggregates in aqueous media were measured by dynamic light

• scattering (DLS). The steady-state and time-resolved photoluminescence spectroscopy studies revealed that all the molecules show intense fluorescence both in solution and in the aggregated state. In THF solutions, a blue emission was observed for the unsubstituted (H), methyl- (Me) and tert-butyl- (t-Bu

Selective recognition of ATP by multivalent nano-assemblies of bisimidazolium amphiphiles through “turn-on” fluorescence response

• Rakesh Biswas,
• Surya Ghosh,
• Shubhra Kanti Bhaumik and
• Supratim Banerjee

Beilstein J. Org. Chem. 2020, 16, 2728–2738, doi:10.3762/bjoc.16.223

• derivatives in aqueous buffer (5 mM tris-HCl, 10 mM NaCl, water/DMSO 99.5:0.5, pH 7.4) were investigated using a combination of absorption and emission spectral studies, dynamic light scattering (DLS) and field emission scanning electron microscopy (FESEM). In a “good solvent” such as DMSO, in which all

• , water/DMSO, 99.5:0.5, pH 7.4). Supporting Information Supporting Information File 382: Synthetic procedures, additional spectroscopic, microscopic and dynamic light scattering data (figures and tables) and NMR characterization. Funding IISER Kolkata and INSPIRE are acknowledged for research

The B & B approach: Ball-milling conjugation of dextran with phenylboronic acid (PBA)-functionalized BODIPY

• Patrizia Andreozzi,
• Lorenza Tamberi,
• Elisamaria Tasca,
• Gina Elena Giacomazzo,
• Marta Martinez,
• Mirko Severi,
• Marco Marradi,
• Stefano Cicchi,
• Sergio Moya,
• Giacomo Biagiotti and
• Barbara Richichi

Beilstein J. Org. Chem. 2020, 16, 2272–2281, doi:10.3762/bjoc.16.188

• by means of UV–vis spectroscopy, Fourier-transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), and transmission electron microscopy (TEM) analysis (see Supporting Information File 1). Concerning the chemical structure of the conjugate, the data reported in the literature were

• emission bands were at λem = 524 nm (λexc = 380 nm) and λem = 523 nm (λexc = 460 nm, see Supporting Information File 1, Figure S2). Then, a Stokes shift of 16 nm could be appreciated in agreement with data previously reported for PBA-BODIPY (1) [26][35]. Dynamic light scattering (DLS) measurements were

• obtain maximum signal intensity, and between each sample, a rinse solution constituted of 2% v/v HNO3 was used to avoid memory effects. Dynamic light scattering (DLS) measurements were carried out with a ζ-Sizer Malvern Instrument in the backscattering mode. All studies were performed at a 173

Targeted photoswitchable imaging of intracellular glutathione by a photochromic glycosheet sensor

• Xianzhi Chai,
• Hai-Hao Han,
• Yi Zang,
• Jia Li,
• Xiao-Peng He,
• Junji Zhang and
• He Tian

Beilstein J. Org. Chem. 2019, 15, 2380–2389, doi:10.3762/bjoc.15.230

• glycosheet which β-D-galactosides pointing away from the surface. Dynamic light scattering (DLS) exhibited a size of 198.6 nm for the 2D MnO2 nanosheets (Figure 2C), which was in accordance with the TEM characterization. The size of the photochromic Glyco-DTE@MnO2 glycosheet was determined as 255.4 nm

Morphology-tunable and pH-responsive supramolecular self-assemblies based on AB₂-type host–guest-conjugated amphiphilic molecules for controlled drug delivery

• Yang Bai,
• Cai-ping Liu,
• Di Chen,
• Long-hai Zhuo,
• Huai-tian Bu and
• Wei Tian

Beilstein J. Org. Chem. 2019, 15, 1925–1932, doi:10.3762/bjoc.15.188

• -assembly and the size of β-CD-BM2-based supramolecular self-assemblies under neutral and acidic pH were first investigated by employing transmission electron microscopy (TEM) and dynamic light scattering (DLS). The prepared β-CD-BM2 were dissolved under neutral pH solution to form FSSAs. TEM revealed that

Synthesis, enantioseparation and photophysical properties of planar-chiral pillar[5]arene derivatives bearing fluorophore fragments

• Guojuan Li,
• Chunying Fan,
• Guo Cheng,
• Wanhua Wu and
• Cheng Yang

Beilstein J. Org. Chem. 2019, 15, 1601–1611, doi:10.3762/bjoc.15.164

• further investigated by dynamic light scattering (DLS) analyses, and revealed the formation of nanoparticles with 615 nm in average diameter after the addition of 50% water (Figure 4d). Increasing the amount of water to 70% or 90% led to a bathochromic shift of the UV–vis absorption and a raise of the

• path length installed in a Unisoku cryostat. Dynamic light scattering (DLS) studies were done on a Zetasizer Nano ZS90 instrument. A preparative Chiralpak IA column was used for the separation of enantiomers. Synthesis and characterization of P5A-DPA The synthesis and characterization data of P5A-Py

Reversible end-to-end assembly of selectively functionalized gold nanorods by light-responsive arylazopyrazole–cyclodextrin interaction

• Maximilian Niehues,
• Patricia Tegeder and
• Bart Jan Ravoo

Beilstein J. Org. Chem. 2019, 15, 1407–1415, doi:10.3762/bjoc.15.140

• photostationary states of the AAP moiety in comparison to azobenzenes used in previous studies. Furthermore, dynamic light scattering was conducted to analyze the assembly in solution (Figure 5). The mean diameter increases upon dAAP addition from ca. 100 nm to ca. 230 nm. Upon UV-light irradiation, the mean

• diameter decreases to 120 nm. We note that for anisotropic particles, the hydrodynamic diameter values give only a rough overestimation of the nanoparticle diameter and are not suitable to characterize end-to-end assembly. However dynamic light scattering clearly confirms the light-responsive aggregation

• by dynamic light scattering. Light-responsive end-to-end assembly of host-functionalized gold nanorods (AuNR) by cyclodextrin–AAP interaction. Two-step ligand exchange reaction for the synthesis of water-soluble cyclodextrin end-functionalized gold nanorods. Supporting Information Supporting

Self-assembly behaviors of perylene- and naphthalene-crown macrocycle conjugates in aqueous medium

• Xin Shen,
• Bo Li,
• Tiezheng Pan,
• Jianfeng Wu,
• Yangxin Wang,
• Jie Shang,
• Yan Ge,
• Lin Jin and
• Zhenhui Qi

Beilstein J. Org. Chem. 2019, 15, 1203–1209, doi:10.3762/bjoc.15.117

• a dynamic light scattering (DLS) measurement (Figure S10, Supporting Information File 1), which was proven to be a convenient method to study aggregation behavior in solvents [98][99]. It was found that the average diameter of aggregates of 1 in CHCl3 (100 μM) was around 31.1 nm. The size of

Dynamic light scattering studies of the effects of salts on the diffusivity of cationic and anionic cavitands

• Anthony Wishard and
• Bruce C. Gibb

Beilstein J. Org. Chem. 2018, 14, 2212–2219, doi:10.3762/bjoc.14.195

• account specific ion–ion interactions. To identify specific ion–ion interactions possibly contributing to the aggregation of proteins, we have used dynamic light scattering (DLS) to probe the aggregation of charged cavitands. DLS measurements of negatively charged 1 in the presence of a range of alkali

• ] (Figure 1), to different salts using dynamic light scattering (DLS) [17][18][19][20]. Respectively functionalized with carboxylates and trimethylammonium groups, these hosts are expected to possess unique ion-pairing properties and hence have very different reverse-Hofmeister responses to added salts

• of 2 result in significant ion-pairing effects that can, in extreme cases such as I− salts, lead to a pronounced reverse Hofmeister effect. Importantly, our DLS studies reveal that this effect is also influenced indirectly by the nature of the cation of the salt. Conclusion Dynamic light scattering

Synthesis of new p-tert-butylcalix[4]arene-based polyammonium triazolyl amphiphiles and their binding with nucleoside phosphates

• Vladimir A. Burilov,
• Guzaliya A. Fatikhova,
• Mariya N. Dokuchaeva,
• Ramil I. Nugmanov,
• Diana A. Mironova,
• Pavel V. Dorovatovskii,
• Victor N. Khrustalev,
• Svetlana E. Solovieva and
• Igor S. Antipin

Beilstein J. Org. Chem. 2018, 14, 1980–1993, doi:10.3762/bjoc.14.173

• scattering (DLS) measurements Dynamic light scattering (DLS) experiments and zeta-potential measurements (ELS) were carried out on a Zetasizer Nano ZS instrument (Malvern Instruments, USA) with 4 mW 633 nm He–Ne laser light source and a light scattering angle of 173°. The data were treated with DTS software

• solutions dried on the surface of the KBr disc. Melting points were measured using a Stuart SMP10 apparatus. UV–vis absorbance spectra The UV–vis-spectra were recorded on a Lambda 35 UV/VIS spectrophotometer (Perkin Elmer Instruments) in an optical cell with 1.0 cm light pass at 298 K. Dynamic light

Carbohydrate inhibitors of cholera toxin

• Vajinder Kumar and
• W. Bruce Turnbull

Beilstein J. Org. Chem. 2018, 14, 484–498, doi:10.3762/bjoc.14.34

• spacing’s to maximize interactions with the target protein. Dynamic light scattering and analytical ultracentrifugation demonstrated that the glycoprotein formed a 1:1 complex with the target CTB protein and was highly effective as an inhibitor with an IC50 value of 104 pM. Multivalent inhibitors with more

Binding abilities of polyaminocyclodextrins: polarimetric investigations and biological assays

• Marco Russo,
• Daniele La Corte,
• Annalisa Pisciotta,
• Serena Riela,
• Rosa Alduina and
• Paolo Lo Meo

Beilstein J. Org. Chem. 2017, 13, 2751–2763, doi:10.3762/bjoc.13.271

• carefully pipetted and the relevant optical activities were measured. Data were finally subjected to regression analysis according to Equation 1 (the relevant mathematical details are reported in Supporting Information File 1). Dynamic light scattering DLS measurements to evaluate the possible dimensions of

Synthesis and supramolecular properties of regioisomers of mononaphthylallyl derivatives of γ-cyclodextrin

• Markéta Bláhová,
• Sergey K. Filippov,
• Lubomír Kováčik,
• Jiří Horský,
• Simona Hybelbauerová,
• Zdenka Syrová,
• Tomáš Křížek and
• Jindřich Jindřich

Beilstein J. Org. Chem. 2017, 13, 2509–2520, doi:10.3762/bjoc.13.248

• light scattering (DLS) [18][19][20][21] and cryo-transmission electron microscopy (Cryo-TEM) [22][23][24]. Results and Discussion Synthesis We present two approaches for the synthesis of naphthylallyl (NA) derivatives. The first approach is a cross-metathesis of peracetylated allyl derivatives of γ-CD

• light scattering (DLS) The supramolecular properties of all regioisomers of NA-γ-CD (2-O-, 3-O-, 6-O-) were investigated by DLS. We analyzed the dependence of self-assembling of the isomers on external stimuli such as agitation and temperature. The 3-O-isomer 2b shows co-existence of two types of

• were studied by a set of methods allowing characterization of supramolecular behavior at various levels – from binary complexation, over supramolecular oligomers to large assemblies; namely by isothermal titration calorimetry (ITC) [16], 1H nuclear magnetic resonance spectroscopy (1H NMR), dynamic

2-Methyl-2,4-pentanediol (MPD) boosts as detergent-substitute the performance of ß-barrel hybrid catalyst for phenylacetylene polymerization

• Julia Kinzel,
• Daniel F. Sauer,
• Marco Bocola,
• Marcus Arlt,
• Tayebeh Mirzaei Garakani,
• Andreas Thiel,
• Klaus Beckerle,
• Tino Polen,
• Jun Okuda and
• Ulrich Schwaneberg

Beilstein J. Org. Chem. 2017, 13, 1498–1506, doi:10.3762/bjoc.13.148

• . Experiments utilizing dynamic light scattering (DLS) revealed an interaction of the PE–PEG micelles with the phenylacetylene, showing a decrease in the size distribution of micelles (Figure S3, Supporting Information File 1). Increasing the MPD concentration (up to c(MPD) = 200 mM) did not lead to a

• III TOF/TOF mass spectrometer (Bruker Daltonics, Billerica, MA, US). GPC was measured on an Agilent Series 1100 (Midland, ON, Canada), equipped with two SDV linear N columns of 8 × 300 mm and 8 × 600 mm measures and 5 µm pore size, in THF at 30 °C against a poly(styrene) standard. Dynamic light

• scattering was performed with a Zetasizer Nano Line (Malvern Instruments, Worcestershire, UK). The rhodium catalyst 2 was synthesized according to literature procedures [12]. Phenylacetylene (3) is commercially available and was used as received. All other chemicals were used as received if not mentioned

Kinetic analysis of mechanoradical formation during the mechanolysis of dextran and glycogen

• Naoki Doi,
• Yasushi Sasai,
• Yukinori Yamauchi,
• Tetsuo Adachi,
• Masayuki Kuzuya and
• Shin-ichi Kondo

Beilstein J. Org. Chem. 2017, 13, 1174–1183, doi:10.3762/bjoc.13.116

• -branched polysaccharide could be measured by dynamic light scattering (DLS), it is difficult to precisely detect a particle with a diameter of less than 10 nm with our experimental setup. The hydrodynamic radius (Rh) is utilized as an index of the spread of a polymer. It is well-known that the Rh of a

• ] = 5,900, 9,600, 21,100, 47,100, 109,000, 200,000, 344,000, and 708,000 g/mol). Dynamic light scattering measurements Analogous to the description in [29], dynamic light scattering was measured using a DLS-5500G Photal dynamic light scattering spectrophotometer (Otsuka Electronics Co., Ltd., Osaka, Japan

Glycoscience@Synchrotron: Synchrotron radiation applied to structural glycoscience

• Serge Pérez and
• Daniele de Sanctis

Beilstein J. Org. Chem. 2017, 13, 1145–1167, doi:10.3762/bjoc.13.114

• highly monodisperse. Besides sample quality control by using complementary analysis, such as dynamic light scattering, native gel, ultracentrifugation, many BioSAXS beamlines at synchrotrons are nowadays equipped with size exclusion chromatography devices directly connected with the sample cell and the

Glyco-gold nanoparticles: synthesis and applications

• Federica Compostella,
• Olimpia Pitirollo,
• Alessandro Silvestri and
• Laura Polito

Beilstein J. Org. Chem. 2017, 13, 1008–1021, doi:10.3762/bjoc.13.100

• thio-amphiphilic linker to impart improved solubility and flexibility to the glycosyl-ligand. UV–vis spectroscopy and dynamic light scattering measurements have been exploited to detect at low picomolar concentrations lactose-AuNP, mannose-AuNP and GlcNAc-AuNP interactions with their cognate lectins

• indicative of the successful binding [85]. In this field, a naturally occurring sialylglycopeptide extracted from egg yolks was converted into a thiol-terminated molecule and grafted on AuNPs, to develop a gold-based sensor for influenza virus detection, using both UV–vis spectroscopy and dynamic light

• scattering. Moreover, a selective detection of the influenza virus can be accomplished. Viral hemagglutinin (HA) protein, in fact, can bind selectively sialic acid residues, but the human influenza virus recognizes the sialic acid α(2,6)galactose sequence while the avian virus recognizes sialic acid α(2,3

Novel β-cyclodextrin–eosin conjugates

• Gábor Benkovics,
• Damien Afonso,
• András Darcsi,
• Szabolcs Béni,
• Sabrina Conoci,
• Éva Fenyvesi,
• Lajos Szente,
• Milo Malanga and
• Salvatore Sortino

Beilstein J. Org. Chem. 2017, 13, 543–551, doi:10.3762/bjoc.13.52

• by dynamic light scattering For the investigation of the aggregation properties, solutions of the eosin–β-CD conjugates in water were used. A concentration of 1 mM of the conjugates Eo-β-CDs was applied to obtain reliably high scattered intensities for the characterization. Figure 5 shows that the

Stabilization of nanosized titanium dioxide by cyclodextrin polymers and its photocatalytic effect on the degradation of wastewater pollutants

• Tamás Zoltán Agócs,
• István Puskás,
• Erzsébet Varga,
• Mónika Molnár and
• Éva Fenyvesi

Beilstein J. Org. Chem. 2016, 12, 2873–2882, doi:10.3762/bjoc.12.286

• agent, carboxymethyl β-cyclodextrin polymer for improving the colloidal stability of nanosized TiO2. Dynamic light scattering measurements proved that the aggregation of TiO2 nanoparticles was hindered by the presence of this polymer additive. Studying its influence on the photocatalytic effect on

• by dynamic light scattering: Determination of the particle size distribution of the nanoTiO2 particles and aggregates was performed by dynamic light scattering method (also known as photon correlation spectroscopy) using a Malvern Zetasizer Nano ZS instrument using a He-Ne laser of 4 mW power and 633

Formose reaction accelerated in aerosol-OT reverse micelles

• Makoto Masaoka,
• Tomohiro Michitaka and
• Akihito Hashidzume

Beilstein J. Org. Chem. 2016, 12, 2663–2667, doi:10.3762/bjoc.12.262

• the cases of AOT and CTAB, the radius of the water pools (Rw) was evaluated by dynamic light scattering, whereas, in the case of TX, Rw was estimated by the fluorescence quenching technique. As can be seen in Supporting Information File 1, Figure S1, Rw for AOT and CTAB is almost proportional to the